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KMID : 0359319980380010139
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Korean Journal of Veterinary Research
1998 Volume.38 No. 1 p.139 ~ p.145
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In vitro isolation of a bovine Neospora in Korea
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김재훈/Kim, Jae Hoon
손현주/김대용/황우석/이병천/황의경/진영화/Sohn, Hyun Joo/Kim, Dae Yong/Hwang, Woo Suk/Lee, Byung Chun/Hwang, Eui Kyung/Jean, Young Hwa
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Abstract
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The Neospora sp. was isolated from the brain of 1 calf via continuous in vitro cultivation in Vero cell. Neospora tachyzoites were observed 45 days after inoculation of the homogenized brain suspension into the Vero cell. The isolated parasite (named tentatively as NCKB-1) was morphologically and ultrastructurally similar to the previously reported Neospora sp isolated in cattle (BPA-1, JPA-1). A comparison of the antigenic reactivity of cultivated tachyzoites with polyclonal antisera to Neospora caninum and Toxoplasma gondii confirmed that this protozoal isolate was similar to N caninum. This is the first report of successful isolation of Neospora sp from cattle in Korea.
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